Keiichi INOUE

氏名

井上 圭一

生年月日 昭和54年4月20日
血液型 A
e-mail inoue.keiichi@nitech.ac.jp
出身地 徳島県
出身高校 兵庫県立小野高等学校
研究テーマ 生体分子の時間分解分光 測定
新規ロドプシンの機能研究
好きなもの
本、角田さん、日本史、
ゆるきゃら、ラーメン
嫌いなもの
生チーズ(天敵)、TG の散乱、
レーザーの不調
長所
物理、化学、生物に経験有り
短所
数学が苦手
特技
きれいなスライド作り
趣味
読書、スポーツ観戦、
動物園& 水族館巡り、旅行
座右の銘
人の一生は、重き荷を負うて
遠き路を行くが如し。 急ぐべからず

小さいことを積み重ねることが
とんでもないところに行く
ただ一つの道
コメント
物 理化学と生物物理を主点に
神取研とロドプシンの
新しいサイエンスを切り拓く

  【原著論文】(査読あり)
50. Functional Characterization of Sodium-pumping Rhodopsins with Different Pumping Properties
Satoshi P. Tsunoda, Matthias Prigge, Rei Abe-Yoshizumi, Keiichi Inoue, Yuko Kozaki, Toru Ishizuka, Hiromu Yawo, Ofer Yizhar, Hideki Kandori*. (2017, July 27) PLOS ONE, 12, issue 7, e0179232

49. Molecular properties of a DTD channelrhodopsin from Guillardia theta
Yumeka Yamauchi, Masae Konno, Shota Ito, Satoshi P. Tsunoda, Keiichi Inoue, and Hideki Kandori*. (2017, May 20) Biophysics and Physicobiology, 14, pp 57-66
doi: 10.2142/biophysico.14.0_57

48. FTIR Analysis of a Light-driven Inward Proton Pumping Rhodopsin at 77 K
Shota Ito, Shinya Sugita, Keiichi Inoue, Hideki Kandori*. (2017, April 5) Photochemistry and Photobiology, in press
doi: 10.1111/php.12771

47. Solid-state NMR Structural Study of the Retinal-binding Pocket in Sodium Ion Pump Rhodopsin
Arisu Shigeta, Shota Ito, Keiichi Inoue, Takashi Okitsu, Akimori Wada, Hideki Kandori, Izuru Kawamura*. (2016, January 1) Biochemistry, 56, issue 4, 543-550
doi: 10.1021/acs.biochem.6b00999

46. A Chimera Na+-pump Rhodopsin as an Effective Optogenetic Silencer
Mohammad Razuanul Hoque, Toru Ishizuka, Keiichi Inoue, Rei Abe-Yoshizumi, Hiroyuki Igarashi, Takaaki Mishima, Hideki Kandori, Hiromu Yawo*. (2016, November 18) PLOS ONE, 11, issue 11, e0166820
doi: 10.1371/journal.pone.0166820

45. Natural Light-driven Inward Proton Pump
Keiichi Inoue, Shota Ito, Yoshitaka Kato, Yurika Nomura, Mikihiro Shibata, Takayuki Uchihashi, Satoshi P. Tsunoda, Hideki Kandori*. (2016, November 17) Nature Communications, 7, Article number:13415
doi: 10.1038/ncomms13415

44. Role of Asn112 in a Light-driven Sodium Ion-pumping Rhodopsin
Rei Abe-Yoshizumi, Keiichi Inoue, Hideaki E. Kato, Osamu Nureki, Hideki Kandori*. (2016, September 27) Biochemistry, 55, issue 41, 5790-5797
doi: 10.1021/acs.biochem.6b00741

43. Primary Photocycle of Na+ Pump Rhodopsin Probed by Baseline-free Femto- to Submillisecond Stimulated Raman
Spectroscopy
Yusaku Hontani, Keiichi Inoue, Miroslav Kloz, Yoshitaka Kato, Hideki Kandori, John T.M. Kennis*. (2016, August 17) Physical Chemistry Chemical Physics, 18, issue 35, 24729-24736
doi: 10.1039/C6CP05240A.

42. Asymmetric Functional Conversion of Eubacterial Light-driven Ion Pumps
Keiichi Inoue, Yurika Nomura, Hideki Kandori*. (2016, May 6)The Journal of Biological Chemistry, 291, issue 19, 9883-9893
doi: 10.1074/jbc.M116.716498jbc.M116.716498.

41. Mutant of a Light-Driven Sodium Ion Pump Can Transport Cesium Ions
Masae Konno, Yoshitaka Kato, Hideaki Kato, Keiichi Inoue, Osamu Nureki, Hideki Kandori*. (2015, December 14) The Journal of Physical Chemistry Letters, 7, issue 1, 51-55
doi: 10.1021/acs.jpclett.5b02385

40. Kinetic Analysis of H+ - Na+ Selectivity in a Light-Driven Na+ Pumping Rhodopsin
Yoshitaka Kato, Keiichi Inoue, Hideki Kandori*. (2015, December 17) The Journal of Physical Chemistry Letters, 6, issue 22, 4481-4486
doi: 10.1021/acs.jpclett.5b02371


39. Ultrafast Photoreaction Dynamics of a Light-driven Sodium-ion-pumping Retinal Protein from Krokinobacter
      eikastus Revealed by Femtosecond Time-resolved Absorption Spectroscopy
Shinya Tahara, Satoshi Takeuchi, Rei Abe-Yoshizumi, Keiichi Inoue, Hiroyuki Ohtani, Hideki Kandori*, Tahei Tahara*. (2015, October 30) The Journal of Physical Chemistry Letters, 6, issue 22, 4481-4486
doi: 10.1021/acs.jpclett.5b01994

38.
A New Group of Eubacterial Light-driven Retinal-binding Proton Pumps with an Unusual Cytoplasmic Proton
      Donor
Andrew Harris, Milena Ljumovic, Ana-Nicoleta Bondar, Yohei Shibata, Shota Ito, Keiichi Inoue, Hideki Kandori*, Leonid S. Brown*. (2015, December) Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1847, issue 12, 1518-1529
doi: 10.1016/j.bbabio.2015.08.003

37. Structural Basis for Na+ Transport Mechanism by a Light-Driven Na+ Pump
Keiichi Inoue, Masae Konno, Rei Abe-Yoshizumi, Hideki Kandori*. (2015, July 24) Angewandte Chemie International Edition, 54, issue 39, 11536-11539
doi: 10.1002/anie.201504549

36. Structural Basis for Na+ Transport Mechanism by a Light-Driven Na+ Pump
Hideaki E. Kato, Keiichi Inoue, Rei Abe-Yoshizumi, Yoshitaka Kato, Hikaru Ono, Masae Konno, Toru Ishizuka, Mohammad Razuanul Hoque, Shoko Hososhima, Hirohumi Kunitomo, Jumpei Ito, Susumu Yoshizawa, Keitaro Yamashita, Mizuki Takemoto, Tomohiro Nishizawa, Reiya Taniguchi, Kazuhiro Kogure, Andr?s D. Maturana, Yuichi Iino, Hiromu Yawo, Ryuichiro Ishitani, Hideki Kandori*, Osamu Nureki*. (2015, May 7) Nature, 521, Number 7550, 48-53
doi: 10.1038/nature14322

35. Converting a Light-driven Proton Pump into a Light-gated Proton Channel
Keiichi Inoue, Takashi Tsukamoto, Kazumi Shimono, Yuto Suzuki, Seiji Miyauchi, Shigehiko Hayashi, Hideki Kandori, Yuki Sudo*. (2015, February 25) Journal of the American Chemical Society, 137, issue 9, pp 3291-3299
doi: 10.1021/ja511788f

34. Na+ Transport by Sodium Ion Pump Rhodopsin is Resistant to Environmental Change -A Comparison of
Photocycles of Na+ and Li+ Transport Processes
Keiichi Inoue, Hikaru Ono, Hideki Kandori*. (2014, December 11) Chemistry Letters, 44, No. 3, 294-296
doi: 10.1246/cl.141023

33. Spectroscopic Study of a Light-Driven Chloride Ion Pump from Marine Bacteria
Keiichi Inoue, Faisal Hammad Mekky Koua, Yoshitaka Kato, Rei Abe-Yoshizumi, Hideki Kandori*. (2014, Aug 28) The Journal of Physical Chemistry B, 118, issue 38, pp 11190-11199
doi: 10.1021/jp507219q

32. FTIR Spectroscopy of a Light-Driven Compatible Sodium Ion-Proton Pumping Rhodopsin at 77 K
Hikaru Ono, Keiichi Inoue, Rei Abe-Yoshizumi, Hideki Kandori*. (2014, May 8) The Journal of Physical Chemistry B, 118, issue 18, pp 4784-4792
doi: 10.1021/jp500756f

31. Chimeric Proton-Pumping Rhodopsins Containing the Cytoplasmic Loop of Bovine Rhodopsin
Kengo Sasaki, Takahiro Yamashita, Kazuho Yoshida, Keiichi Inoue, Yoshinori Shichida, Hideki Kandori*. (2014, March 12) PLOS ONE, 9, issue 3, e91323
doi: 10.1371/journal.pone.0091323

30. Thermal and Spectroscopic Characterization of a Proton Pumping Rhodopsin from an Extreme Thermophile
Takashi Tsukamoto, Keiichi Inoue, Hideki Kandori, and Yuki Sudo*. (2013, June 5) The Journal of Biological Chemistry, 288, issue 30, pp 21581-21592
doi: 10.1074/jbc.M113.479394

29. A Blue-Shifted Light-driven Proton Pump for Neural Silencing
Yuki Sudo*, Ayako Okazaki, Hikaru Ono, Jin Yagasaki, Seiya Sugo, Motoshi Kamiya, Louisa Reissig, Keiichi Inoue, Kunio Ihara, Hideki Kandori, Shin Takagi and Shigehiko Hayashi. (2013, May 28) The Journal of Biological Chemistry, 288, issue 28, pp 20624-20632
doi: 10.1074/jbc.M113

28. Role of Trimer-trimer Interaction of Bacteriorhodopsin Studied by Optical Spectroscopy and High-speed Atomic
Force Microscopy
Hayato Yamashita, Keiichi Inoue, Mikihiro Shibata, Takayuki Uchihashi, Jun Sasaki, Hideki Kandori, Toshio Ando*. (2013) Journal of Structural Biology, 184, 2-11
doi: 10.1016/j.jsb.2013.02.011

27. A Light-driven Sodium Ion Pump in Marine Bacteria
Keiichi Inoue, Hikaru Ono, Rei Abe-Yoshizumi, Susumu Yoshizawa, Hiroyasu Ito, Kazuhiro Kogure, Hideki Kandori*. (2013, Apr.) Nature Communications, 4, Article number:1678
doi: 10.1038/ncomms2689

26. Absorption Spectra and Photochemical Reactions in a Unique Photoactive Protein, Middle Rhodopsin MR
Keiichi Inoue, Louisa Reissig, Makoto Sakai, Shiori Kobayashi, Michio Homma, Masaaki Fujii, Hideki Kandori, Yuki Sudo*. (2012, May) Journal of Physical Chemistry B, 116, issue 20, pp 5888-5899 
doi: 10.1021/jp302357m

25. L105K Mutant of Proteorhodopsin
Tushar Kanti Maiti, Keisuke Yamada, Keiichi Inoue, Hideki Kandori*. (2012, Apr. 17) Biochemistry, 51, issue 15, pp 3198-3204
doi: 10.1021/bi201916a

24. Transient Dissociation of the Transducer Protein from Anabaena Sensory Rhodopsin Concomitant with Formation
of the M state Produced upon Photoactivation
Masato Kondoh, Keiichi Inoue, Jun Sasaki, John L. Spudich, Masahide Terazima*. (2011) Journal of American Chemical Society, 133, issue 34, pp 13406-13412

23. Spectrally Silent Intermediates during the Photochemical Reactions of Salinibacter Sensory Rhodopsin I
Keiichi Inoue*, Yuki Sudo, Michio Homma, Hideki Kandori. (2011, Apr.) Journal of Physical Chemistry B, 115, issue 15, pp 4500-4508

22. Chimeric Microbial Rhodopsins Containing the Third Cytoplasmic Loop of Bovine Rhodopsin
Aya Nakatsuma, Takahiro Yamashita, Kengo Sasaki, Akira Kawanabe, Keiichi Inoue, Yuji Furutani, Yoshinori Shichida, Hideki Kandori*. (2011) Biophysical. Journal, 100, issue 8, pp1874-1882

21. Dual Emission Caused by Ring Inversion Isomerization of a 4-Methyl-2-pyridyl-pyrimidine Copper(I) Complex   
Michihiro Nishikawa, Kuniharu Nomoto, Shoko Kume*, Keiichi Inoue, Makoto Sakai, Masaaki Fujii, Hiroshi Nishihara*. (2010) Journal of American Chemical Society, 132, issue 28, pp 9579-9581

20. Infrared Imaging of an A549 Cultured Cell by a Vibrational Sum-frequency Generation Detected Infrared
Super-resolution Microscope   
Satoshi Kogure, Keiichi Inoue, Tsutomu Ohmori, Miya Ishihara, Makoto Kikuchi, Masaaki Fujii*, Makoto Sakai*. (2010) Optics Express, 18, issue 13, pp 13402-13406

19. 蛍光検出を利用した赤外超解像顕微鏡法によるA549細胞の測定
Infrared Super-Resolution Imaging of a Single A549 Cell by Fluorescence Detection
大森努、井上圭一、酒井 誠、藤井正明、石原美弥、菊地眞. 日本レーザー医学会誌, 30, No. 4, pp 427-434

18. Development of a Non-scanning VSFG detected IR Super-resolution Microscope and its Application to
Biological Cells   
Keiichi Inoue, Masaaki Fujii*, Makoto Sakai*. (2010. Mar.) Applied Spectroscopy, 64, Number 3, pp 275-281

17. Spectroscopic Studies of a Sensory Rhodopsin I Homologue from the Archaeon Haloarcula vallismortis
Jin Yagasaki, Daisuke Suzuki, Kunio Ihara, Keiichi Inoue, Takashi Kikukawa, Makoto Sakai, Masaaki Fujii, Michio Homma, Hideki Kandori*, Yuki Sudo*. (2010) Biochemistry, 49, issue 6, pp 1183-1190

16. Visible-super-resolution Infrared Microscopy Using Saturated Transient Fluorescence Detected Infrared
Spectroscopy   
N?ndor Bokor*, Keiichi Inoue, Satoshi Kogure, Masaaki Fujii, Makoto Sakai. (2010) Optics Communications, 283, issue 3, pp 509-514

15. Characterization of a Signaling Complex Composed of Sensory Rhodopsin I and Its Cognate Transducer Protein
from the Eubacterium Salinibacter ruber   
Yuki Sudo*, Akiko Okada, Daisuke Suzuki, Keiichi Inoue, Hiroki Irieda, Makoto Sakai, Masaaki Fujii, Yuji Furutani, Hideki Kandori, Michio Homma. (2009) Biochemistry, 48, issue 42, pp 10136?10145

14. Two-point-separation in a Sub-micron Nonscanning IR Super-resolution Microscope Based on Transient
Fluorescence Detected IR Spectroscopy   
Keiichi Inoue, N?ndor Bokor, Satoshi Kogure, Masaaki Fujii*, Makoto Sakai*. (2009) Virtual Journal for Biomedical Optics, 4, issue 9, pp 12013

13. Two-point-separation in a Sub-micron Nonscanning IR Super-resolution Microscope Based on Transient
Fluorescence Detected IR Spectroscopy   
Keiichi Inoue, N?ndor Bokor, Satoshi Kogure, Masaaki Fujii*, Makoto Sakai*. (2009) Optics Express, 17, issue 14, pp 12013-12018

12. Effects of Chloride Ion Binding on the Photochemical Properties of Salinibacter Sensory Rhodopsin I   
Daisuke Suzuki, Yuji Furutani, Keiichi Inoue, Takashi Kikukawa, Makoto Sakai, Masaaki Fujii, Hideki Kandori, Michio Homma, Yuki Sudo*. (2009) Journal of Molecular Biology, 392, issue 1, pp 48-62

11. Reaction Dynamics of Halorhodopsin Studied by Time-Resolved Diffusion   
Keiichi Inoue, Megumi Kubo, Makoto Demura, Naoki Kamo, Masahide Terazima*. (2009) Biophysical Journal, 96, issue 9, pp 3724-3734

10. Functional Imaging of a Single Cell: Far-field Infrared Super-resolution Microscopy Using Auto-fluorescence
Detection   
Tsutomu Ohmori, Keiichi Inoue, Makoto Sakai, Masaaki Fujii, Miya Ishihara, Makoto Kikuchi. (2009. Feb.) Proceedings of SPIE, 7182, 71820G-1-6

  9. Synthesis of Pd Complexes Directly Linked to the Light-Absorbing [(bpy)3Ru]2+ Unit and Their Photochemical
Reactions toward Styrenes    
Akiko Inagaki*, Hiroki Nakagawa, Munetaka Akita*, Keiichi Inoue, Makoto Sakai, and Masaaki Fujii. (2008) Dalton Transactions, issue 47, pp 6709-6723

  8. Signal Transmission through the HtrII Transducer Alters the Interaction of Two alpha-Helices in the HAMP Domain
Keiichi Inoue, Jun Sasaki, John L. Spudich, Masahide Terazima* (2008) Journal of Molecular Biology, 376, issue 4, pp 963-970

  7. Energetics and Role of the Hydrophobic Interaction during Photoreaction of the BLUF Domain of AppA
Partha Hazra, Keiichi Inoue, Wouter Laan, Klaas J. Hellingwerf and Masahide Terazima* (2008. Feb.) Journal of Physical Chemistry B, 112, issue 5, pp 1494-1501

  6. Photoreverse Reaction Dynamics of Octopus Rhodopsin
Keiichi Inoue, Motoyuki Tsuda and Masahide Terazima* (2007. May.) Biophysical Journal, 92, issue 10, pp 3643-3651

  5. Laser-Induced Transient Grating Analysis of Dynamics of Interaction between Sensory Rhodopsin II D75N and
the HtrII Transducer
Keiichi Inoue, Jun Sasaki, John L. Spudich, Masahide Terazima* (2007. Mar.) Biophysical Journal, 92, issue 6, pp 2028-2040

  4. Tetramer Formation Kinetics in the Signaling State of AppA Monitored by Time-Resolved Diffusion
Partha Hazra, Keiichi Inoue, Wouter Laan, Klaas J. Hellingwerf, Masahide Terazima* (2006. Jul.) Biophysical Journal, 91, issue 2, pp 654-661

  3. Diffusion Coefficient and the Secondary Structure of Poly-L-glutamic Acid in Aqueous Solution
Keiichi Inoue, Naoki Baden, Masahide Terazima* (2005. Dec.) Journal of Physical Chemistry B, 109, issue 47, pp 22623-22628

  2. Time-Resolved Enthalpy Changes of Sensory Rhodopsin II and the Transducer Complex during Photo-Reaction
Keiichi Inoue, Jun Sasaki, Masayo Morisaki, Fumio Tokunaga, Masahide Terazima* (2005. Jun.) Journal de Physique IV France, 125, pp 769-772

  1. Time-Resolved Detection of Sensory Rhodopsin II-Transducer Interaction
Keiichi Inoue, Jun Sasaki, Masayo Morisaki, Fumio Tokunaga, Masahide Terazima* (2004) Biophysical Journal, 87, issue 4, pp 2587-2597

  【総説】(査読あり)
  6. The Light-driven Sodium Ion Pump: A New Player in Rhodopsin Research
Hideaki E. Kato*, Keiichi Inoue, Hideki Kandori, Osamu Nureki* (2016. Nobemver 17) Bioessays, 38, issue 12, pp 1274-1285
doi: 10.1016/j.tim.2014.10.009.

  5. The Study and Application of Photoreceptive Membrane Protein, Rhodopsin
Keiichi Inoue (2016. August 22) Bulletin of the Chemical Society of Japan, 89, pp 1416-1424
doi: 10.1246/bcsj.20160235.

  4. The Study on a Novel Light-driven Sodium Pump and Creation of New Functional Molecules
Keiichi Inoue (2016. August) Molecular Science, 10, Number 1, AA0086 (Invited review)
doi: 10.3175/molsci.10.A0086.

  3. Light-driven Ion-translocating Rhodopsins in Marine Bacteria
Keiichi Inoue, Yoshitaka Kato, Hideki Kandori* (2015. February) Trends in Microbiology, 23, issue 2, pp 91-98
doi: 10.1016/j.tim.2014.10.009.

  2. Molecular and Evolutionary Aspects of Microbial Sensory Rhodopsins
Keiichi Inoue, Takashi Tsukamoto, Yuki Sudo* (2014. May) Biochimica et Biophysica Acta (BBA) - Bioenergetics, 87, issue 5, pp 2587-2597
doi: 10.1016/j.bbabio.2013.05.005

  1. IR Super-resolution Microspectroscopy and Its Application to Single Cells
Makoto Sakai*, Keiichi Inoue, Masaaki Fujii* (2013. February) Current Pharmaceutical Biotechnology, 14, issue 2, pp 159-166

  【特許】
  1. プロトンポンプおよびその利用
      神取 秀樹, 井上 圭一. 特願2016-173556 (2016, September 6)

  【受賞】
  8. 26th IUPAC International Symposium on Photochemistry, Awards for excellent presentations: Young researcher oral
      presentation awards, Photochemical & Photobiological Sciences (2016)
Title: “Spectroscopic and structural studies on light-driven sodium pump rhodopsin”

  7. 第65回日本化学会進歩賞(平成27年度)
業績題目「光受容膜タンパク質ロドプシンの光化学の 基礎研究と応用」

  6.
第 8回分子科学会奨励賞
業績題目「光駆動ナトリウムポンプの発見とメカニズ ム研究および機能創 成」

  5. 日 本化 学会第95春季年会(2015) 優秀講演賞(学術)
業績題目「ナトリウムポンプ型ロドプシンのナト リウム輸送メカニズム についての分光研究」

  4. 分 子科学研究奨励森野基金(平成26年度)
業績題目「先端的分光法によるロドプシンタ ンパク質の光誘起機能 発現メカニズムの解明」

  3. 平 成 26年度 文部科学大臣表彰・若手研究者賞
業績題目「微生物型ロドプシンの物理化 学研究」

  2. 平 成25年度 公益財団法人 光科学技術研究振興財団 研究表彰
業績題目「光駆動型ナトリウムポン プの発見」

  1. 日 本化学会第93春季年会(2013)・若い世代の特別講演会
業績題目「物理化学的アプロー チによる新奇光駆動型 Na+/H+ハイブリッ ドポンプロドプシンの研究」